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Table 3 Characteristics of DCs from different donors following wash-out of maturation cocktails and cryopreservation

From: Generation of clinical grade dendritic cells with capacity to produce biologically active IL-12p70

Parameter   DC1 DC2 DC3 DC4 DC5
Wash-out       
% viable cells N* 5 5 4 5 2
  m+ 93.4 81.4 84.9 87.4 80.2
  std# 4.2 4.2 5.0 3.6 4.8
% CD14+ cells N* 5 5 4 4 2
  m+ 0.9 4.7 0.8 2.3 3.6
  std# 1.0 7.3 0.4 3.3 4.7
% CD83+ cells N* 5 5 4 5 2
  m+ 91.7 79.3 86.5 86.1 88.2
  std# 3.0 8.4 6.8 6.5 8.2
% CCR7+cells N* 5 5 4 5 2
  m+ 89.4 71.4 81.7 86.1 76.1
  std# 4.3 15.1 6.6 4.6 6.9
Cryopreservation       
% viable cells N* 3 2 2 3 2
  m+ 86.5 60.6 81.8 84.5 64.2
  std# 4.0 4.4 2.1 1.8 14.9
% CD14+ cells N* 3 2 2 3 2
  m+ 1.6 0.4 0.5 1.9 1.8
  std# 2.2 0.3 0.2 2.5 2.1
% CD83+cells N* 3 3 2 3 2
  m+ 95.8 84.9 97.5 96.1 95.3
  std# 2.1 7.3 0.0 1.1 3.9
% CCR7+ cells N* 3 2 2 3 2
  m+ 81.3 76.3 80.7 81.6 84.5
  std# 6.6 7.4 2.5 6.3 0.2
% CD80+cells N* 3 2 2 3 2
  m+ 80.6 71.6 87.8 90.4 82.7
  std# 5.6 3.8 4.4 0.9 3.7
% CD86+ cells N* 3 2 2 3 2
  m+ 96.3 94.8 96.9 96.0 93.5
  std# 2.7 0.8 0.9 1.4 6.8
  1. * N = number of independent donors
  2. + m = mean
  3. #std = standard deviation