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Table 3 Characteristics of DCs from different donors following wash-out of maturation cocktails and cryopreservation

From: Generation of clinical grade dendritic cells with capacity to produce biologically active IL-12p70

Parameter

 

DC1

DC2

DC3

DC4

DC5

Wash-out

      

% viable cells

N*

5

5

4

5

2

 

m+

93.4

81.4

84.9

87.4

80.2

 

std#

4.2

4.2

5.0

3.6

4.8

% CD14+ cells

N*

5

5

4

4

2

 

m+

0.9

4.7

0.8

2.3

3.6

 

std#

1.0

7.3

0.4

3.3

4.7

% CD83+ cells

N*

5

5

4

5

2

 

m+

91.7

79.3

86.5

86.1

88.2

 

std#

3.0

8.4

6.8

6.5

8.2

% CCR7+cells

N*

5

5

4

5

2

 

m+

89.4

71.4

81.7

86.1

76.1

 

std#

4.3

15.1

6.6

4.6

6.9

Cryopreservation

      

% viable cells

N*

3

2

2

3

2

 

m+

86.5

60.6

81.8

84.5

64.2

 

std#

4.0

4.4

2.1

1.8

14.9

% CD14+ cells

N*

3

2

2

3

2

 

m+

1.6

0.4

0.5

1.9

1.8

 

std#

2.2

0.3

0.2

2.5

2.1

% CD83+cells

N*

3

3

2

3

2

 

m+

95.8

84.9

97.5

96.1

95.3

 

std#

2.1

7.3

0.0

1.1

3.9

% CCR7+ cells

N*

3

2

2

3

2

 

m+

81.3

76.3

80.7

81.6

84.5

 

std#

6.6

7.4

2.5

6.3

0.2

% CD80+cells

N*

3

2

2

3

2

 

m+

80.6

71.6

87.8

90.4

82.7

 

std#

5.6

3.8

4.4

0.9

3.7

% CD86+ cells

N*

3

2

2

3

2

 

m+

96.3

94.8

96.9

96.0

93.5

 

std#

2.7

0.8

0.9

1.4

6.8

  1. * N = number of independent donors
  2. + m = mean
  3. #std = standard deviation
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Journal of Translational Medicine

ISSN: 1479-5876

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