Figure 5
From: CMV pp65 and IE-1 T cell epitopes recognized by healthy subjects
Identification of CMV pp65 15-mer and nanomer epitopes from peptide subpool 8 that were reactive with CD8+ T cells. Testing of IE-1 15-mers from subpool 8 against cells from donor 2 (gold bar), donor 5 (red bar), donor 6 (light blue bar), and donor 7 (navy blue bar) revealed that the peptide IE-1297–311 was reactive with donor 6, IE-1305–319 was reactive with donor 7, IE-1309–323 was reactive with donors 2 and 6, and IE-1313–327 was reactive with donor 5 (Panel A). Testing of the 6 nanomers overlapping IE-1297–311 against cells from donor 6 revealed that IE-1300–308 was the dominant nanomer (Panel B). Testing of the nanomers spanning the overlapping 15-mers IE-1305–319, IE-1309–323, and IE-1313–327 against PBMCs from donors 2, 5, and 7 identified 4 nanomers that were reactive with CD8+ T cells: IE-1305–313, IE-1308–316, IE-1312–320 and IE-1319–327, (Panel C).