Figure 4
From: CMV pp65 and IE-1 T cell epitopes recognized by healthy subjects
Identification of CMV IE-1 15-mer and nanomer epitopes from 3 peptide subpools reactive with CD8+ T cells. PBMCs from 4 donors were reactive with peptides in IE-1 subpools 3, 5, and 10. Testing of all 15-mers in each subpool identified 3 nanomers reactive with CD8+ T cells, one from each subpool. Testing of the 6 overlapping nanomers spanning each reactive 15-mer lead to the identification of 3 nanomer epitopes.