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Figure 3 | Journal of Translational Medicine

Figure 3

From: CMV pp65 and IE-1 T cell epitopes recognized by healthy subjects

Figure 3

Identification of CMV pp65 15-mer and nanomer epitopes recognized by CD8+ T cells. PBMCs from CMV-seropositive subjects reactive with peptides in a pp65 subpool were tested against all the individual 15-mers in each subpool. Following the identification of reactive 15-mers, nanomers overlapping at 8 amino acids and spanning the reactive 15-mer peptides were tested against PBMCs from the reactive subject. The reactive 15-mer and nanomer peptides are shown. Testing of cells from 7 subjects led to the identification of 6 pp65 15-mers reactive with CD8+ T cells. Testing of the overlapping nanomers identified 5 epitopes. Cells from donor 3 were not available to test with the nanomer peptides. Although CD8+ T cells from donor 10 were reactive with peptides in subpool 6 and a reactive 15-mer, pp65221–235, was identified, no reactive nanomer was identified. NT = not tested.

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