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Figure 2 | Journal of Translational Medicine

Figure 2

From: CMV pp65 and IE-1 T cell epitopes recognized by healthy subjects

Figure 2

Identification of CMV IE-1 epitopes using 15-mer and nanomer peptides. PBMCs were stimulated with a library of 120 15-mer peptides overlapping by 11 residues and covering the entire IE-1 protein. IFN-γ flow cytometry was used to detect T cell responses. To identify specific epitopes, PBMCs were stimulated with peptide subpools containing 10 overlapping 15-mer peptides. PBMCs from donors with reactive CD8+ T cells were tested further with individual 15-mers and nanomers. The results of analysis of CD8+ T cell responses in donor 6 to IE-1 peptides are shown.

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