Table 1 Primer sequences used for RT-PCR
From: Tumor-specific T cells signal tumor destruction via the lymphotoxin β receptor
Gene | Forward primer sequence | Reverse primer sequence |
|---|---|---|
MIP-1α | 5'-atg aag gtc tcc acc act gcc ctt g-3' | 5'-ggc att cag ttc cag gtc agt gat-3' |
MIP-1β | 5'-gtt ctc agc acc aat ggg ctc tga-3' | 5'-ctc tcc tga agt ggc tcc tcc tg-3' |
IP-10 | 5'-cct atc ctg ccc acg tgt tg-3' | 5'-cgc acc tcc aca tag ctt aca-3' |
RANTES | 5'-cat cct cac tgc agc cgc c-3' | 5'-cca agc tgg cta gga cta gag-3' |
MIG | 5'-atg aag tcc gct gtt ctt ttc-3' | 5'-tta tgt agt ctt cct tga acg ac-3' |
HPRT | 5'-gtt gga tac agg cca gac ttt gtt g-3' | 5'-gag ggt agg atg gcc tat agg ct-3' |