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Figure 3 | Journal of Translational Medicine

Figure 3

From: Tumor-specific T cells signal tumor destruction via the lymphotoxin β receptor

Figure 3

Expression of Chemokine by D5 melanoma cells and Chemotaxis of Macrophages In Vitro. (A) Macrophage chemotactic activity of D5 supernatant after incubation with effector T cells. Supernatant derived from cultured D5 tumor cells or D5 tumor cells that were cocultured with effector T cells for 24 hours were plated in the lower chamber of a transwell plate. The number of DJ2P macrophage cells placed on the upper chambers that trans-migrated into the lower chamber was determined by FACS analysis with FITC-labeled antiCD11b antibody. (B) Chemokine expression by D5 tumor cells or effector T cells with and without stimulation. Expression of chemokines by D5 tumor cells after cocluture with effector T cells and removal of T 24 hours late or effector T cells stimulated with anti-CD3 antibody for 24 hours were analyzed by RT-PCR. HPRT expression was used for the control of total mRNA.

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