Supervised Eisen hierarchical clustering analysis of 133 differentially expressed genes between pre and post wound that were not included in the signature clusters. From the 753 differentially expressed genes, the genes in the signature clusters were removed and genes important in inflammation, immune response and wound healing were selected according to DAVID  and Gene Cards  and analyzed by supervised hierarchical clustering. Three clusters were identified; one whose expression was increased most on day 2, one on days 4 and 8, and another whose expression was increased on day 2–8. The specific genes are shown in additional file 2. Pre and post wound biopsies are color coded by the horizontal bars as per figure 1.