Figure 8

Poly-ICLC stimulates GL261 glioma-expression of IFN-beta, TLR3, MHC class I and IP-10. In vitro cultured GL261 cells were treated with or without 50 μg/ml poly-ICLC for 24 hrs, and evaluated for expression of IFN-β in culture-supernatant by specific ELISA (A), surface TLR3 (B) and H-2K^b (C) by flow-cytometry, and for production of IP-10 in culture-supernatant by specific ELISA (D). (B), Dashed thin line: isotype control antibody, Thin line: TLR3 expression without poly-ICLC treatment, Bold line: TLR3 expression in the presence of poly-ICLC. (C), Black thin line: isotype control antibody, Dashed thin line: H-2K^b expression without poly-ICLC treatment, Filled grey area: H-2K^b expression with poly-ICLC treatment and control isotype-IgG, Black thick line: H-2K^b expression with poly-ICLC treatment and neutralizing anti IFN-β mAb.