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Figure 1 | Journal of Translational Medicine

Figure 1

From: Toll like receptor-3 ligand poly-ICLC promotes the efficacy of peripheral vaccinations with tumor antigen-derived peptide epitopes in murine CNS tumor models

Figure 1

High-level expression of EphA2 is restricted to GL261 glioma in the mouse brain. Paraffin embedded tissue sections prepared from the brains of C57BL/6 mice bearing day 14 GL261 glioma in the right frontal lobe were stained with anti-EphA2 monoclonal antibody (C-20 Ab; Santa Cruz Biotechnology, Inc). After washing, sections were incubated with biotinylated goat anti-rabbit IgG (Vector Laboratories), followed by avidin-biotin-complex peroxidase (Vectastain ABC kits; Vector Laboratories). Reaction products were developed using a Nova Red substrate kit (Vector Laboratories) giving rise to red-brown deposits. The sections were also counter-stained with hematoxylin (blue). The letter "T" in the figure indicates tumor tissue, with the letter "N" in the figure indicating normal brain tissue. Original magnification; × 20.

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