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Table 1 Percentage of c-mpl+ cells in human HSC/PC differentiation stages

From: The thrombopoietin receptor, c-Mpl, is a selective surface marker for human hematopoietic stem cells

 

PERCENTAGE c-mpl+

Stage I

Stage II

Stage III

Stage IV

Tissue Source

#

 

Manual Gate

Overton Algorithm

SED Algorithm

Manual Gate

Overton Algorithm

SED Algorithm

Manual Gate

Manual Gate

FL

5

Mean

71.54

77.23

86.43

50.76

60.19

72.09

10.15

3.40

  

S.D.

10.48

8.02

5.94

14.94

12.04

11.24

5.99

3.49

UCB

5

Mean

67.18

76.24

83.82

49.92

65.83

75.63

12.85

7.91

  

S.D.

18.55

11.11

9.55

21.59

19.74

18.91

12.51

13.43

ABM

5

Mean

81.96

85.66

92.25

61.60

69.87

80.06

5.93

0.58

  

S.D.

16.29

13.06

9.69

23.94

21.76

19.37

3.93

0.61

mPBSC

4

Mean

71.40

77.58

85.05

48.25

57.14

66.85

7.87

0.17

  

S.D.

14.55

13.25

9.29

22.86

19.74

17.39

4.53

0.07

  1. ABM, adult bone marrow; FL, fetal liver; mPBSC, cytokine-mobilized peripheral blood stem cells; SD, standard deviation; SED, super-enhanced Dmax subtraction; UCB, umbilical cord blood