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Figure 1 | Journal of Translational Medicine

Figure 1

From: The thrombopoietin receptor, c-Mpl, is a selective surface marker for human hematopoietic stem cells

Figure 1

The most primitive CD34+ HSC/PC from all major tissue sources express the highest levels of c-mpl surface receptor. A. Representative CD34-FITC vs. CD38-PE contour plots generated from magnetically selected viable CD34+ mononuclear cells from representative specimens of the four major tissue sources of human HSC/PC: FL, UCB, ABM, and mPBSC. Gates defining the four differentiation stages of human CD34+HSC/PC as defined by Terstappen et al. [6] are shown: I: CD34+38-. II: CD34+38dim III: CD34+38+ IV: CD34dim38+. B. Overlay of the c-mpl-APC histogram plots of viable human CD34+ mononuclear cells from the four HSC/PC differentiation stages depicted in Fig. 1A above. Stage I [red]. Stage II [blue]. Stage III [green]. Stage IV [light blue]. For comparison, a control histogram plot [gray] is shown that is generated from differentiation stage I cells stained with an IgG1 isotype control-APC antibody equivalent in concentration to the c-mpl-APC antibody stain. Isotype control histogram plots for each HSC/PC differentiation stage were used to separately set the manual gates that define the c-mpl+ and c-mpl-- cell populations for each stage. C. CD34-FITC vs. CD38-PE dot plots derived from the same representative human CD34+ tissue specimens as depicted in Fig. 1A. From each specimen, all cell events were initially categorized by their percentile level of fluorescence on the c-mpl-APC parameter on a log histogram plot. Three gates were drawn on the histogram plot to define three discrete levels of c-mpl expression: High (98–100 percentile); Intermediate (48–50 percentile); Low (18–20 percentile). Viable cell events falling within one of these three percentile ranges are depicted on the dot plot with their corresponding color code: High [light blue]; Intermediate [dark blue]; Low [red]. D. Same data as C, but using contour plots to visualize the data. High [red]; Intermediate [yellow]; Low [blue].

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