Ascites spheroids and mesothelial cell monolayers are viable for 7 days. Ascites spheroids from eight patients were plated on confluent mesothelial cell monolayers in 96-well plates for seven days. At days 0, 1, 4, and 7, wells were stained with Annexin V-FITC and propidium iodide. Panels shows confluent mesothelial monolayers alone (lef column) or with patient ascites sample 3 at day 4 (middle column) or day 7 (right column) with a bright-field image (row 1), Annexin V-FITC staining (row 2), and propidium iodide staining (row 3). Arrows delineate the perimeters of ascites spheroids that have attached and spread on the mesothelial cell monolayer. Magnification = 100×.