Up-regulation of HLA class-I antigen expression and antigen-specific CTL response in cervical cancer cells by the demethylating agent hydralazine and the histone deacetylase inhibitor valproic acid

Table 1 Primers and conditions used for PCR and RT-PCR analysis.

Primer set	Sense 5'-3'	Antisense 5'-3'	Size pb	Annealing
MHC-I promoter	ccagttcagggacagagattacggg	gagagggagaaaagaaactgcggag	280	60°C
HLA-A	gacagcgacgccgcgagcca	ggcagcgaccacagctccag	807	64°C
HLA-B	gacagcgacgccgcgagtcc	agtagcgaccacagctccga	907	60°C
HLA-C	gagatcacactgacctggca	gaacacagtcagtgtgggg	589	53°C
β-actin	gggtcagaaggattcctatg	ggtctcaaacatgatctggg	238	58°C
E7HPV16	cagctcagaggaggaggatg	gcccattaacaggtcttcca	166	60°C
E6HPV18	atgctgcatgccataaatgt	tgcccagctatgttgtgaaa	214	60°C
actin	acacctggacctggactcac	gctcttggctcctttgtcac	231	60°C
MY09	cgtccmarrggawactgatc			45°C
MY11		gcmcagggwcataayaatgg	450

ISSN: 1479-5876