Figure 6

HPV-16 E7 and HPV-18 E6 gene expression in CaSki and MS751 cell lines treated with H and VA. Total RNA was extracted from CaSki and MS751 cell lines treated with either H, VA or H plus VA and then RT-PCR analysis for the HPV-16 E7 and HPV-18 E6 gene expression in CaSki and MS751 was respectively performed using E7 and E6 primer pairs (Table 1). The effect of human recombinant IFN-γ and the sum of H/VA/IFN-γ was also tested.