Figure 2

Transcriptional effect of hydralazine and valproic acid upon expression of HLA class-I molecules. Total RNA was extracted from C33A, CaSki and MS751 cells treated with H, VA or H/VA. RT-PCR analysis was performed using HLA-A, -B or -C and β-actin primer pairs (Table 1). PCR products were then separated on a 3% agarose gel. One hundred-bp markers (MW) were run in the flanking lanes of each gel. Human recombinant IFN-gamma was used as a positive inducer of the HLA class I expression. RNA of the peripheral blood lymphocytes (PBL) from a normal donor, was used as a positive control. A is C33A, B is Caski and C corresponds to MS751 cell lines. Changes in expression as determined by densitometric analysis according to treatments are described in results.