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Figure 1 | Journal of Translational Medicine

Figure 1

From: Detection of human MCP-4/CCL13 isoforms by SELDI immunoaffinity capture

Figure 1

SELDI immunoaffinity capture of MCP-4/CCL13 from patient sera after high dose IL-2 immunotherapy. Anti MCP-4/CCL13 clone 18 (A-I, O), anti MCP-4/CCL13 clone 20 (Q,R) and anti IL-6 (L-N) polyclonal antibody were immobilized on a protein G array. Spectra from 6000 to 10000 Da are depicted for each experiment. No significant peaks were detected out of this range. Immunoaffinity capture spectra of patient 2(A), 3(B), 5(C) serum diluted 1:1 in PBS. Immunoaffinity capture spectra of MCP-4/CCL13 immunoprecipitated from patient 2(D), 3(E), 5(F). (G) Immunoaffinity capture spectra of the un-bound fraction (flow-through) obtained during the immunoprecipitation procedure of patient 2(G), 3(H), 5(I). Immunoaffinity capture spectra of MCP-4/CCL13 immunoprecipitated from patient 2(L), 3(M), 5(N) serum on IL-6 pre-coated chip. (O) Immunoaffinity capture spectra of PBS. (P) spectra of patient 2 serum applied on an uncoupled PG20 chip. Immunoaffinity capture spectra of MCP-4/CCL13 immunoprecipitated from patient 2(Q) and 3(R) serum on MCP-4/CCL13 clone 20 pre-coated chip. Intensity scale for A and P spectra = 0 – 10. Intensity scale for Q and R = 0 – 2. Red bar = MCP-4/CCL13 clone 18 pre-coated chip; orange bar = MCP-4/CCL13 clone 20 pre-coated chip; blue bar = IL-6 pre-coated chip; dotted bar = Uncoupled PG20 chip.

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