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Figure 6 | Journal of Translational Medicine

Figure 6

From: Characterization of highly frequent epitope-specific CD45RA+/CCR7+/- T lymphocyte responses against p53-binding domains of the human polyomavirus BK large tumor antigen in HLA-A*0201+ BKV-seropositive donors

Figure 6

Phenotype and functions of LTag 579/HLA-A*0201 multimer-labeled specific T cells "ex vivo". PBMCs from three HLA-A*0201+ BKV LTag seropositive and one HLA-A*0201+ BKV LTag seronegative donors were stained with LTag 579/HLA-A*0201-multimers and the ex vivo CD8+ T cell phenotype was analyzed. Panel A shows frequencies of specific LTag 579-HLA-A*0201 multimer positive cells plotted against CD8+ T cells: cells were considered positive if their mean fluorescence intensities exceeded by ten fold that of multimer negative CD8+ cells. LTag 579/HLA-A*0201+ cells were also evaluated for their CD45RA and CCR7 surface expression. Panel B reports the expression of IL-2, IL-4, and IL-10 genes by "ex vivo" peptide induced PBMCs from the same donors. Data are reported as fold increase of specific gene expression (relative quantification, 2-ΔΔCt method), as compared to unstimulated cultures.

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