Figure 1

Effects of various concentrations of isoflurane on metabolic activity of candidal yeast culture. Actively growing yeast cultures were treated with various concentrations of isoflurane (0.05% 0.8% v/v of media) and amphotericin B (4 μg/ml). The top of each syringe was tightly sealed to create semi-anaerobic environment. The metabolic activity of each syringe was determined by volume of displacement (an indicator of metabolic activity of Candidal yeast) in the syringe. Panel A shows volume of displacement in syringes (Smaller arrows indicate the levels) whereas Panel B is graphic representation of the displaced volume. Columns in the graph represent the means (+/- Standard error of the mean (SEM)) of four independent experiments.