Figure 5
From: A novel assay for analysis of the regulation of the function of human osteoclasts
Short-term effects of resorption-inhibitors on release of CTX-I by human osteoclasts. When bone resorption was underway, as judged from inspection of sample bone slices, bone slices were washed and transferred to new wells containing fresh medium containing M-CSF/RANKL. After 2 h of incubation this supernatant was removed for CTX-I assay, and replaced with medium containing M-CSF/RANKL, together with test reagents or vehicle. Cultures were incubated for a further 1 h. This supernatant was then itself removed for assay, and the process repeated with further incubation periods of 1 and 2 h. CTX-I release was calculated as nM released per hour, and expressed as a percentage of that released per hour before the test periods. CTX-I release before test period (nM): controls: 22 ± 2.5; salmon CT (10 ng/ml): 35 ± 12; MV061194 3 × 10-7 M): 34 ± 13. n = 4 cultures per group.