Figure 2From: A novel assay for analysis of the regulation of the function of human osteoclastsQuantification of bone resorption on individual bone slices in the same experiment. A single preparation of PBMCs was added to multiple bone slices. Bone slices were incubated in different wells with M-CSF and RANKL for 10 days. At the end of the culture period cells were removed, and the area of each bone slice excavated by osteoclasts was measured.Back to article page