Figure 6
Tumor challenge in optimal vaccine dose conditions. C3 challenge of vaccinated and control mice (n = 8) with E7-expressing C3 cells. C57BL/6 mice (n = 8) were immunized with 100 μg E7 peptide emulsified using vortex, syringe-extrusion or homogenizer for ten minutes and boosted two weeks later. Ten days after the boost, the mice were challenged with 5 × 105 C3 cells. The control mice developed tumors but all the vaccinated mice successfully rejected the C3 cells. The mean tumor volume and standard error of the mean of each group of mice over time is shown. Figure shown is representative of four experiments.