Figure 5
in vivo cytotoxicity assay. C57BL/6 mice (n = 4) were immunized with E7 peptide emulsified using vortex, syringe-extrusion or homogenizer for ten minutes and boosted two weeks later. Control mice (n = 4) were vaccinated with PBS:IFA emulsified by syringe-extrusion for ten minutes without peptide. One week after the boost, the mice were sacrificed and their spleens harvested. Figure is a box-plot showing the five-number summary (median, maximum, minimum, 25th and 75th quartile) of the % specific lysis of E7 peptide-pulsed target cells after subtracting background killing of irrelevant peptide control PSA peptide-pulsed cells collected over five experiments. Median percentage specific lysis is 13% for the vortex group, 17% for the syringe-extrusion group and 21% for the homogenizer group.