Figure 4

IFN-γ ELISPOT. C57BL/6 mice (n = 4) were immunized with E7 peptide emulsified using vortex, syringe-extrusion or homogenizer for ten minutes and boosted two weeks later. One week after the boost, the mice were sacrificed and their lymph nodes harvested. E7peptide-specific response was measured by IFN-γ secreted after subtracting background IFN-γ secretion in the presence of PSA peptide as an irrelevant control. Control mice (n = 4) were vaccinated with PBS:IFA emulsion without peptide. The mean and standard error of the mean of each group is shown below. Figure shown is representative of three experiments. A Student's t-test (one-tailed) showed that there was a significant difference between the vortex versus the syringe-extrusion technique (p = 0.04) but no statistically significant differences between the homogenizer versus the vortex technique (p = 0.17) or the homogenizer technique versus the syringe-extrusion technique (p = 0.17).