Functional expression of a single-chain antibody to ErbB-2 in plants and cell-free systems

Table 1 Reactivity of ScFv 800E6 with ErbB-2 transfectants and human cell lines

Cell line	Lineage	CTVαScFv	ScFv800E6	mAb 800E6	source/ref
32D	Myelomonocyte Leukemia (murine)	7¹	7	7	[21]
32D-ErbB-2	Myelomonocyte Leukemia (transfectants)	6	171	2430
SK-BR-3		4	278	2256	ATCC/[21]
MDA-MB-361		3	60	399
ZR-75-1	Breast Ca	2	47	289
DAL1		8	44	155	our lab
MCF7		3	19	76	ATCC/[21]
T47D		2	18	50
End 9	Endometrial Ca	2	2	21	our lab
CRL1	Melanoma	6	7	10
BE	Melanoma	1	2	20	[21]
ES-1	Melanoma	0	2	22
A431	Epidermoid Ca	0	0	10
SKOV3	Ovary Ca	1	1	10

¹Mean fluorescence intensity (m.f.i.) values estimated by flow cytometry after subtraction of background staining in the presence of PBS + anti murine Ig-FITC (background m.f.i. ranged from 5 in 32D cells to 15 in the other cell lines). Cut-off levels can be arbitrarily set at values twice the highest background, e.g. above 15.

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