Figure 6
From: Functional expression of a single-chain antibody to ErbB-2 in plants and cell-free systems
Immunohistochemistry of breast carcinoma lesions with different preparations of ScFv800E6. Semi-serial cryostatic sections of a primary ductal breast carcinoma lesion were stained with (A) 50 μl of His(6x)-N-ScFv800E6 transcription-translation mixes; (B) 50 μl of extracts from plants transiently expressing ScFv800E6; (C) 50 μl of extracts from wild-type plants; and (D) 50 μl of mAb 800E6 (50 μg/ml) in PBS/5% FCS. ScFv/mAb binding was revealed as described in Materials and Methods.