Figure 1

Cloning strategy and map of ScFv 800E6 constructs. Diagram illustrating the construction of ScFvs for expression in plants (stable and transient expression: panels A and B, respectively), and in cell-free transcription-translation systems (C–G). A linker peptide sequence (Gly₄Ser)₃, multiple cloning sites (MCS), the T7 promoter, the IPTG-inducible pLac promoter, a 3' transcription terminator, a proteolytic cleavage site (Xa), a ribosome binding site (RBS), the Strep II and His-tags, the kanamycin resistance NPT II gene, the 35S-CaMV promoter, the NOS terminator region for transcript stabilization, the RNA-dependent RNA-polymerase binding site (RdRb), the viral movement proteins (M1, M2 and M3), and the viral coat protein (CP) are present in different plasmids. A synopsis of the different ScFvs and tag positions is provided in panel H.