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Table 1 Chemical shifts of 15N-labelled residues in Dsg49-60REWVKFAKPCRE peptide, and changes on control, PV or PF Aab addition

From: Proteomic definition of a desmoglein linear determinant common to Pemphigus vulgaris and Pemphigus foliaceous

15N-residue1

Chemical Shift Values:

        
 

Theoretical2:

Experimental3:

      
 

Free peptide

Free peptide

Control Aabs

+PV Aabs

+PF Aabs

 

1HN

15N

1HN

15N

1HN

15N

1HN

15N

1HN

15N

1-Arg4

6.78

77.6

6.95

80.6

7.02

80.7

7.24

81.2

-

-

4-Val

8.65

120.8

8.18

122.7

8.18

122.5

8.16

122.5

-

-

6-Phe

8.42

121.7

7.97

119.3

7.97

119.0

7.96

119.0

-

-

7-Ala

8.29

122.3

8.40

120.0

8.40

120.0

8.38

119.8

-

-

11-Arg4

6.78

77.6

7.22

81.4

7.27

81.6

7.20

81.6

-

-

 

8.14

119.6

8.14

117.9

8.13

117.7

8.12

117.9

-

-

  1. 115N-amino acid position in the peptide. 2 Theoretical chemical shift values were derived from bmrb.wisc.edu, and corrected for deviations due to local sequence effects [27]. 3 Experimental values from resonance spectra reported in Fig. 4. 4 Average value of chemical shifts relative to the H and N atoms of η amino residues in arginine. The chemical shifts relative to the H and N atoms of α amino residue are undetectable in 1-Arg because of the NH2 terminal position.