Figure 4
1H-15N NMR HSQC spectra and relative 1-D contour plots of the low- redundancy Dsg349–60R EWV KFA KPCR E peptide 15N-labelled at residues 1, 4, 6, 7 and 11. A) free peptide in solution; plus AAbs from B) prostate cancer, C) PF, or D) PV patients. Upper panels: portions of the HSQC spectra showing resonances from residues 1, 4, 6, 7 and 11. Lower panels: expanded region of the HSQC spectra showing resonances from the residues 4, 6, 7 and 11, by being 1-Arg at terminal position. 15N-labelled residues are given underlined.