Table 3 Isolated total RNA mass and volume, mass of cDNA after PCR amplification and the corresponding data for RNA amplified by in vitro transcription together with amplification factors for each step and overall amplification
Pt No. | Total RNA volume (μL) | Total RNA mass (μg) | Amplified cDNA (μg) | PCR amplification factor | Amplified RNA volume (μL) | [Amplified RNA] (μg/μL) | Transcription amplification factor | Overall amplification factora |
|---|---|---|---|---|---|---|---|---|
1 | 40 | 15.7 | 63.5 | 15.9 | 400 | 31.1 | 195 | 3100 |
2 | 90 | 30.4 | 74.0 | 18.5 | 1000 | 2.1 | 27 | 500 |
3 | 100 | 10.4 | 51.9 | 9.9 | 2013 | 1.8 | 70 | 700 |
4 | 35 | 15.8 | 75.1 | 10.7 | 1471 | 2.2 | 44 | 500 |
5 | 155 | 78.9 | 63.2 | 9.0 | 1320 | 5.2 | 109 | 1000 |
6 | 40 | 20.2 | 99.0 | 14.1 | 1592 | 1.7 | 37 | 500 |
Avg | 76.67 | 28.6 | 71.1 | 13.0 | 1299 | 7.4 | 81 | 1100 |
SD | 47.75 | 25.5 | 16.1 | 3.8 | 552 | 11.7 | 63 | -- |