Preparing clinical-grade myeloid dendritic cells by electroporation-mediated transfection of in vitro amplified tumor-derived mRNA and safety testing in stage IV malignant melanoma

Table 2 Primer sequences and predicted amplicon sizes in detection of melanoma-specific transcripts in dendritic cells

Transcript	Sequence	Predicted amplicon size (bp)	Ref.
GAPDH	GAA GGT GAA GGT CGG AGT C	226	[29]
GAPDH	GAA AGA TGG TGA TGG GAT TC
Mart1 Out1	ATG CCA AGA GAA GAT GCT CAG	384
Mart1 Out2-2	AGC ATG TCT CAG GTG TCT CG
gp100 Out1	GCT TGG TGT CTC AAG GCA ACT	751	[30]
gp100 Out2-2	CTC CAG GTA AGT ATG AGT GAC
Tyrosinase Out1	TTG GCA GAT TGT CTG TAG CC	284
Tyrosinase Out2-2	AGG CAT TGT GCA TGC TGC TT

ISSN: 1479-5876