Figure 3
Electrotransfected dendritic cells can express transfected mRNA. IDCs, 1.0 × 107 cells/mL, were electroporated by two 1.0-kV/cm 50-μs pulses separated by 500 ms in the presence of 20 μg eGFP-mRNA per 1.0 × 106 cells, matured for three days when fluorescence due to eGFP was quantified by flow cytometry. Dotted line: control cells electroporated without mRNA; full line, the cells electroporated in the presence of eGFP-mRNA. Shown is a histogram typical of four experiments.