Figure 2
Identifying conditions for electrotransfection of immature dendritic cells. Normal IDCs were electrotransfected with in vitro transcribed eGFP-mRNA. Following electrotransfection, the cells were matured for 48 hours when viability (open symbols) and transfection efficiency (closed symbols) were quantified (by 7-AAD exclusion and eGFP fluorescence, respectively). Shown are the data from the final iteration in the analysis where mRNA concentration varied from 4.0 μg/mL to 25 μg/mL (A), pulse amplitude from 0.5 kV/cm to 2.5 kV/cm (B) and pulse width from 0.05 μs to 0.45 μs (C). Symbols denote mean values of measurements in cells from three or more individuals ± standard deviation (except in panel C that is an example of an entire experiment conducted with cells from one individual).