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Figure 1 | Journal of Translational Medicine

Figure 1

From: Preparing clinical-grade myeloid dendritic cells by electroporation-mediated transfection of in vitro amplified tumor-derived mRNA and safety testing in stage IV malignant melanoma

Figure 1

(A) Size distribution of nucleic acids obtained during processing. Nondenaturing agarose gel electropherograms showing the size distribution of total RNA extracted from the tumor (R), cDNA after reverse transcription (C), amplified cDNA (aC) and in vitro transcribed RNA (iR). rL stands for RNA marker ladder (gel 3) and N for negative control. Upper arrow at the DNA marker ladder (dL) points to the band of 3 kb and the lower arrow to the band of 1 kb. Numbers refer to patients 1 to 6. (B) Capillary microelectropherograms of total RNA isolated from tumor tissue (upper row) and the corresponding in vitro transcribed RNA (lower row). RNA samples from patient 2 are represented on the left and samples from patient 6 on the right. The sharp peak at the left of each plot is a loading control. The two other prominent peaks in the upper plots represent ribosomal RNA.

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