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Table 2 Gene expression studies: dissected aorta ascendens vs. control aorta. Gene expression studies that comparing freshly dissected aorta ascendens (D) and undissected control aorta (C). 3 different gene expression studies were performed: 16 samples were studied with BD Atlas™ Human1.2 Array (h), 16 samples – with BD Atlas™ Human Cardiovascular Array (c) and 8 samples – with Affymetrix Human Genome U133A GeneChip. All differently expressed genes of the Atlas-experiments, 49 genes (h) and 33 genes (c), are shown and compared to genes of the Affymetrix experiment. Ratio: mean expression of dissected aorta (D) divided by mean expression of control (C); n.e.: not expressed, n.s.: difference not significant. n.t.: not tested, ↓ downregulated, ↑ upregulated; ? – regulation in the Atlas and Affymetrix experiment different

From: Gene expression in acute Stanford type A dissection: a comparative microarray study

Gene Name Platform Atlas Affymetrix regulation
Functional group Array type ratio D/C P value ratio D/C P value
extracellular matrix proteins,       
cell surface antigens, adhesion proteins       
collagen IV α2, COL4A2 h 0.5 <0.01 0.4 <0.05
collagen IV α5, COL4A5   n.t.   0.32 <0.05
collagen VI α3, COL6A3 c 1.9 <0.05   n.s. ?
collagen VIII α1, COL8A1 c 0.3 <0.01 0.3 <0.001
collagen VIII α2, COL8A2   n.t.   0,4 <0,01
collagen XIV α1, COL14A1, undulin c 0.4 <0.001 0.5 <0.001
collagen XVIII α1, COL18A1   n.t.   0.47 <0.001
polycystin1, PKD1 h 0.3 <0.001 0.4 <0.01
polycystin 2, PKD2   n.t.   0.4 <0.001
clusterin, SP-40; h 0.5 <0.01 0.5 <0.01
integrin α7B, IGA7B h 0.4 <0.001 0.3 <0.001
gap junction protein, connexin 43 c 0.6 <0.05   n.s. ?
SPARC-like 1 c 0.3 <0.001 0.6 <0.001
decorin c 0.3 <0.05   n.s.
elastin   n.t.   0.4 <0.05
fibulin 5   n.t.   0.4 <0.001
cell surface glycoprotein MUC18 h 0.3 <0.001 0.5 <0.01
fibronectin receptor h n.s.   3.4 <0.01
ras homolog gene family member B h 0.5 <0.05   n.s. ?
extracellular matrix proteolysis       
MMP-11 h 2.1 <0.001 3.2 <0.05
MMP-14   n.t.   2.5 <0.01
MMP-19   n.t.   3.2 <0.05
TIMP1 h,c 1.8 <0.01   n.s. ?
TIMP2 h,c 0.4 <0.01   n.s. ?
TIMP3 c 0.2 <0.001   n.s. ?
cell motility and cytoskeleton proteins       
vinculin c 0.2 <0.001   n.s. ?
actinin α1 c 0.5 <0.001 0.8 <0.01
filamin A c 0.3 <0.001 0.5 <0.001
actin α2   n.t.   0.13 <0.001
actinin α4   n.t.   0.44 <0.001
gelsolin   n.t.   0.5 <0.01
leiomodin   n.t.   0.4 <0.01
tropomyosin 1   n.t.   0.4 <0.01
calponin   n.t.   0.3 <0.001
smooth & non-muscle myosin light chain kinase h 0.4 <0.001 n.t.  
smooth muscle myosin heavy chain, SM2   n.t.   0.5 <0.001
myosin regulatory light chain2, smooth muscle   n.t.   0.5 <0.001
metabolism   n.t.     
low-density lipoprotein receptor, LDLR c 0.4 <0.001 1.6 <0.01 ?
Kunitz-type serine protease inhibitor 2, SPINT2 h 0.2 <0.001 0.3 <0.001
aldehyde dehydrogenase 2, mitochondrial c 0.3 <0.01 0.6 <0.01
diaphorase (NADH), cytochrome b-5 reductase c 0.5 <0.01   n.s. ?
superoxide dismutase 3, extracellular c 0.4 <0.01 0.4 <0.001
lipase A c 2.6 <0.05   n.s. ?
protease inhibit, PI1, alpha-1-antitrypsin h 5.5 <0.001   
GABA-B receptor 1A subunit h 1.8 <0.001 n.e.   ?
prostaglandin I2 prostacyclin synthetase c 0.4 <0.05 0.81 <0,001
Gene Name Platform Atlas Affymetrix regulation
Functional group Array type ratio D/C P value ratio D/C P value ratio D/C
Protein turnover       
purine-rich element-binding protein A h 0.4 <0.01 0.6 <0.05
glutathione S-transferase theta 1 h 0.4 <0.01 0.2 <0.05
58-kDa inhibit. of RNA-activated protein kinase h 0.3 <0.01   
G1/S-specific cyclin D1 h 0.5 <0.01   n.s. -
carboxypeptidase H, CPE; enkephalin convertase h 0.4 <0.01   
ribos. protein S6 kinase II α1, S6KII-alpha 1 h 3.1 <0.001   n.s. -
carboxypeptidase N, polypeptide 1, 50 kD c 2.0 <0.05 n.e.   -
eukariotic translation initiantion factor 4E bind prot 1   n.t.   2.9 <0.01
60 S acdic ribosomal protein PO   n.t.   3.0 <0.01
FOS-related antigen 1 h,c 4.9 <0.01 3.6 <0.05
myc proto-oncogene h 3.5 <0.01 2.8 <0.05
ets domain protein elk-3; NET; SRF accessory protein h 2.1 <0.001 4.4 <0.05
adenine phosphoribosyltransferase   n.t.   2.1 <0.001
high mobility group prot isoforms I & Y, HMGIY h 10.1 <0.001 6,0 <0.01
superoxide dismutase 2, mitochondrial c 2.6 <0.01 3.7 <0.001
Cell receptors       
Natriuretic peptide receptor A/guanylate cyclase A c 0.4 <0.001 0.3 <0.001
natriuretic peptide receptor C/guanylate cyclase C c 0.5 <0.001   n.s. -
protein tyrosine phosphatase receptor type F c,h 0.4 <0.01 n.t.   -
growth factors       
vascular endothelial growth factor, VEGF h 4.7 <0.05   n.s. -
endothelin 2 c 0.6 <0.01 n.e.   -
fibroblast growth factor receptor1 h 0.6 <0.01 0.7 <0.05
insulin-like growth factor-binding protein 2, IGFBP2 h 0.3 <0.001 0.2 <0.01
cardiotrophin 1 c 0.4 <0.001 0.5 >0.05
pleiotrophin, heparin-binding growth factor 8, HBGF8 h 0.5 <0.05 0.6 <0.05
inflammation + stress response       
colony-stimulating factor 1 receptor, CSF1R h 2.0 <0.05 1.7 <0.05
myeloid cell nuclear differentiation antigen h 3.5 <0.001 3.9 <0.05
tumor necrosis fact recept superfam memb 1B h 2.0 <0.001   n.s. -
granulocyte colony stimul factor recept h 3.5 <0.01 n.e.   -
interleukin 2 receptor alpha subunit, IL-2, CD25 antigen h 3.1 <0.001 5.3 <0.001
interleukin 6, IL-6 h 3.9 <0.01 4.3 <0.001
interleukin 8, IL-8     12.2 <0.05
small inducible cytokine subfamily A member 2 h,c 3.8 <0.01 2.2 <0.05
90-kDa heat-shock proteinA HSP90A h 2.6 <0.05 n.t.  
60-kDa heat shock protein HSP60 h 2.7 <0.05 n.t.  
CD9 antigen p24 c 0.5 <0.001 0.5 <0.01
27-kDa heat shock protein HSP27 h 0.4 <0.001 n.t.   -
blood, hemostasis       
endothelial plasminogen activator inhibit 1, PAI1 h 2.8 <0.05 4.0 <0.05
thrombomodulin h 2.8 <0.05   n.s. -
hexabrachion, tenascin C c 2.6 <0.01 6.2 <0.05
heme oxygenase 1 h 15.4 <0.05 3.6 <0.05
tissue factor pathway inhibit, coagulation inhibit c 2.0 <0.05 1.6 <0.05
tissue factor pathway inhibitor 2 c 2.3 <0.05 3.0 <0.05
others       
c-fgr proto-oncogene p55-FGR h 2.6 <0.01 2.6 <0.01
erbB3 proto-oncogene; HER3 h 3.7 <0.001 n.t.  
caspase 4 h 1.9 <0.05 n.t.  
follistatin-related protein h 0.5 <0.01 0.6 <0.01
Gem; induced immediate early protein h 0.2 <0.001 0.3 <0.001
parathymosin athymosin h 0.6 <0.05 0.8 n.s. -
prostaglandin I2 (prostacyclin) synthase c 0.4 <0.05 n.t. <0.001
calcium-activated potassium channel beta subunit h 0.3 <0.001 0.4 <0.01
solute carrier fam 4 anion exchanger member 3 c 0.5 <0.001 n.t.   -