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Table 2 Gene expression studies: dissected aorta ascendens vs. control aorta. Gene expression studies that comparing freshly dissected aorta ascendens (D) and undissected control aorta (C). 3 different gene expression studies were performed: 16 samples were studied with BD Atlas™ Human1.2 Array (h), 16 samples – with BD Atlas™ Human Cardiovascular Array (c) and 8 samples – with Affymetrix Human Genome U133A GeneChip. All differently expressed genes of the Atlas-experiments, 49 genes (h) and 33 genes (c), are shown and compared to genes of the Affymetrix experiment. Ratio: mean expression of dissected aorta (D) divided by mean expression of control (C); n.e.: not expressed, n.s.: difference not significant. n.t.: not tested, ↓ downregulated, ↑ upregulated; ? – regulation in the Atlas and Affymetrix experiment different

From: Gene expression in acute Stanford type A dissection: a comparative microarray study

Gene Name

Platform

Atlas

Affymetrix

regulation

Functional group

Array type

ratio D/C

P value

ratio D/C

P value

extracellular matrix proteins,

      

cell surface antigens, adhesion proteins

      

collagen IV α2, COL4A2

h

0.5

<0.01

0.4

<0.05

collagen IV α5, COL4A5

 

n.t.

 

0.32

<0.05

collagen VI α3, COL6A3

c

1.9

<0.05

 

n.s.

?

collagen VIII α1, COL8A1

c

0.3

<0.01

0.3

<0.001

collagen VIII α2, COL8A2

 

n.t.

 

0,4

<0,01

collagen XIV α1, COL14A1, undulin

c

0.4

<0.001

0.5

<0.001

collagen XVIII α1, COL18A1

 

n.t.

 

0.47

<0.001

polycystin1, PKD1

h

0.3

<0.001

0.4

<0.01

polycystin 2, PKD2

 

n.t.

 

0.4

<0.001

clusterin, SP-40;

h

0.5

<0.01

0.5

<0.01

integrin α7B, IGA7B

h

0.4

<0.001

0.3

<0.001

gap junction protein, connexin 43

c

0.6

<0.05

 

n.s.

?

SPARC-like 1

c

0.3

<0.001

0.6

<0.001

decorin

c

0.3

<0.05

 

n.s.

elastin

 

n.t.

 

0.4

<0.05

fibulin 5

 

n.t.

 

0.4

<0.001

cell surface glycoprotein MUC18

h

0.3

<0.001

0.5

<0.01

fibronectin receptor

h

n.s.

 

3.4

<0.01

ras homolog gene family member B

h

0.5

<0.05

 

n.s.

?

extracellular matrix proteolysis

      

MMP-11

h

2.1

<0.001

3.2

<0.05

MMP-14

 

n.t.

 

2.5

<0.01

MMP-19

 

n.t.

 

3.2

<0.05

TIMP1

h,c

1.8

<0.01

 

n.s.

?

TIMP2

h,c

0.4

<0.01

 

n.s.

?

TIMP3

c

0.2

<0.001

 

n.s.

?

cell motility and cytoskeleton proteins

      

vinculin

c

0.2

<0.001

 

n.s.

?

actinin α1

c

0.5

<0.001

0.8

<0.01

filamin A

c

0.3

<0.001

0.5

<0.001

actin α2

 

n.t.

 

0.13

<0.001

actinin α4

 

n.t.

 

0.44

<0.001

gelsolin

 

n.t.

 

0.5

<0.01

leiomodin

 

n.t.

 

0.4

<0.01

tropomyosin 1

 

n.t.

 

0.4

<0.01

calponin

 

n.t.

 

0.3

<0.001

smooth & non-muscle myosin light chain kinase

h

0.4

<0.001

n.t.

 

smooth muscle myosin heavy chain, SM2

 

n.t.

 

0.5

<0.001

myosin regulatory light chain2, smooth muscle

 

n.t.

 

0.5

<0.001

metabolism

 

n.t.

    

low-density lipoprotein receptor, LDLR

c

0.4

<0.001

1.6

<0.01

?

Kunitz-type serine protease inhibitor 2, SPINT2

h

0.2

<0.001

0.3

<0.001

aldehyde dehydrogenase 2, mitochondrial

c

0.3

<0.01

0.6

<0.01

diaphorase (NADH), cytochrome b-5 reductase

c

0.5

<0.01

 

n.s.

?

superoxide dismutase 3, extracellular

c

0.4

<0.01

0.4

<0.001

lipase A

c

2.6

<0.05

 

n.s.

?

protease inhibit, PI1, alpha-1-antitrypsin

h

5.5

<0.001

  

GABA-B receptor 1A subunit

h

1.8

<0.001

n.e.

 

?

prostaglandin I2 prostacyclin synthetase

c

0.4

<0.05

0.81

<0,001

Gene Name

Platform

Atlas

Affymetrix

regulation

Functional group

Array type

ratio D/C

P value

ratio D/C

P value

ratio D/C

Protein turnover

      

purine-rich element-binding protein A

h

0.4

<0.01

0.6

<0.05

glutathione S-transferase theta 1

h

0.4

<0.01

0.2

<0.05

58-kDa inhibit. of RNA-activated protein kinase

h

0.3

<0.01

  

G1/S-specific cyclin D1

h

0.5

<0.01

 

n.s.

-

carboxypeptidase H, CPE; enkephalin convertase

h

0.4

<0.01

  

ribos. protein S6 kinase II α1, S6KII-alpha 1

h

3.1

<0.001

 

n.s.

-

carboxypeptidase N, polypeptide 1, 50 kD

c

2.0

<0.05

n.e.

 

-

eukariotic translation initiantion factor 4E bind prot 1

 

n.t.

 

2.9

<0.01

60 S acdic ribosomal protein PO

 

n.t.

 

3.0

<0.01

FOS-related antigen 1

h,c

4.9

<0.01

3.6

<0.05

myc proto-oncogene

h

3.5

<0.01

2.8

<0.05

ets domain protein elk-3; NET; SRF accessory protein

h

2.1

<0.001

4.4

<0.05

adenine phosphoribosyltransferase

 

n.t.

 

2.1

<0.001

high mobility group prot isoforms I & Y, HMGIY

h

10.1

<0.001

6,0

<0.01

superoxide dismutase 2, mitochondrial

c

2.6

<0.01

3.7

<0.001

Cell receptors

      

Natriuretic peptide receptor A/guanylate cyclase A

c

0.4

<0.001

0.3

<0.001

natriuretic peptide receptor C/guanylate cyclase C

c

0.5

<0.001

 

n.s.

-

protein tyrosine phosphatase receptor type F

c,h

0.4

<0.01

n.t.

 

-

growth factors

      

vascular endothelial growth factor, VEGF

h

4.7

<0.05

 

n.s.

-

endothelin 2

c

0.6

<0.01

n.e.

 

-

fibroblast growth factor receptor1

h

0.6

<0.01

0.7

<0.05

insulin-like growth factor-binding protein 2, IGFBP2

h

0.3

<0.001

0.2

<0.01

cardiotrophin 1

c

0.4

<0.001

0.5

>0.05

pleiotrophin, heparin-binding growth factor 8, HBGF8

h

0.5

<0.05

0.6

<0.05

inflammation + stress response

      

colony-stimulating factor 1 receptor, CSF1R

h

2.0

<0.05

1.7

<0.05

myeloid cell nuclear differentiation antigen

h

3.5

<0.001

3.9

<0.05

tumor necrosis fact recept superfam memb 1B

h

2.0

<0.001

 

n.s.

-

granulocyte colony stimul factor recept

h

3.5

<0.01

n.e.

 

-

interleukin 2 receptor alpha subunit, IL-2, CD25 antigen

h

3.1

<0.001

5.3

<0.001

interleukin 6, IL-6

h

3.9

<0.01

4.3

<0.001

interleukin 8, IL-8

   

12.2

<0.05

small inducible cytokine subfamily A member 2

h,c

3.8

<0.01

2.2

<0.05

90-kDa heat-shock proteinA HSP90A

h

2.6

<0.05

n.t.

 

60-kDa heat shock protein HSP60

h

2.7

<0.05

n.t.

 

CD9 antigen p24

c

0.5

<0.001

0.5

<0.01

27-kDa heat shock protein HSP27

h

0.4

<0.001

n.t.

 

-

blood, hemostasis

      

endothelial plasminogen activator inhibit 1, PAI1

h

2.8

<0.05

4.0

<0.05

thrombomodulin

h

2.8

<0.05

 

n.s.

-

hexabrachion, tenascin C

c

2.6

<0.01

6.2

<0.05

heme oxygenase 1

h

15.4

<0.05

3.6

<0.05

tissue factor pathway inhibit, coagulation inhibit

c

2.0

<0.05

1.6

<0.05

tissue factor pathway inhibitor 2

c

2.3

<0.05

3.0

<0.05

others

      

c-fgr proto-oncogene p55-FGR

h

2.6

<0.01

2.6

<0.01

erbB3 proto-oncogene; HER3

h

3.7

<0.001

n.t.

 

caspase 4

h

1.9

<0.05

n.t.

 

follistatin-related protein

h

0.5

<0.01

0.6

<0.01

Gem; induced immediate early protein

h

0.2

<0.001

0.3

<0.001

parathymosin athymosin

h

0.6

<0.05

0.8

n.s.

-

prostaglandin I2 (prostacyclin) synthase

c

0.4

<0.05

n.t.

<0.001

calcium-activated potassium channel beta subunit

h

0.3

<0.001

0.4

<0.01

solute carrier fam 4 anion exchanger member 3

c

0.5

<0.001

n.t.

 

-