Figure 4

Relative expression (Log₂ CY5/Cy3 ratio) of CISH (A), SOCS-2 (B) and scatter plot of their expression in baseline conditions (blue circles) and in response to rIL-2 (red circles) (C). Relative expression of BCL-2 and related genes (D); the p₂-values refer to a two-tailed paired t test. Light blue and red horizontal bars underline PBMC sample from Caucasian and Chinese donors; in separate panels CD4 (small red horizontal bar) and CD8 T cells (light blue bar) exposed to rIL-2 in the presence of PBMC and subsequently isolated are compared with CD4 and CD8 T cells (orange and dark blue horizontal bars respectively) purified before exposure to rIL-2. Ratios are displayed according to the central method for display using a normalization factor [92]. The average expression of BCL-2 and BCL-2L1 is shown of PBMC and individual T cell subsets in the graph below.