Figure 2

A – Clusterogram of genes associated with receptor function significantly modulated by rIL-2 obtained by pair wise t test. Light blue and red horizontal bares underline PBMC sample from Caucasian and Chinese donors; in separate panels CD4 (small red horizontal bar) and CD8 T cells (light blue bar) exposed to rIL-2 in the presence of PBMC and subsequently isolated are compared with CD4 and CD8 T cells (orange and dark blue horizontal bars respectively) purified before exposure to rIL-2. Distinct signatures are labeled in italic; B – mRNA expression of different sub-units of the IL-2R and the IL-7R without (white bars) or following stimulation (blue filled bars) with 300 IU/ml of rIL-2. Significance refers to a pair wise t test. C – Expression of the IL-2Ra chain in CD4 and CD8 T cells stimulated with rIL-2 in the presence of bystander PBMC (average ± SEM of six independent experiments, p2-values refer to a paired two-tailed t test between samples exposed to rIL-2 or non stimulated); D – Expression of the IL-7Ra chain in CD4 and CD8 T cells stimulated with rIL-2 in the presence of bystander PBMC (average ± SEM of six independent experiments, p2-values refer to a paired two-tailed t test between samples exposed to rIL-2 or non stimulated); E Concentration IL-4, IL-7, IL-9 and IL-15 (pg/ml, average of 47 donor samples) in the supernatant of PBMC cultures containing (rIL-2) or not containing (No Stim) 300 IU/ml of rIL-2. Supernatants were harvested after 24 hours. Statistical significance refers to a pair wise t test;