Figure 2

XPE augments immunity of B16 lysate "vaccine". XPE and B16 melanoma cell lysates were prepared as described in Figure 1 using freeze-thaw cycles. C57/BL6 mice were immunized with XPE alone or co-mixed with the B16 melanoma lysate diluted in PBS at a concentration of 2 mg/ml, and injections of 50 uL (total mass 10 ug). Tumor growth was assessed every 3 days by two measurements of perpendicular diameters by a caliper, and animals were sacrificed when tumors reached a size of 1 cm in any direction. *p < 0.05, Student's T test compared to saline treated.