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Figure 3 | Journal of Translational Medicine

Figure 3

From: Clonal analysis of palmar fibromatosis: a study whether palmar fibromatosis is a real tumor

Figure 3

Clonal analysis by the pattern of X-chromosome inactivation (representative cases: cases 3, 4, 7, 9, 12, and positive control). The presence of multiple bands of each allele in the positive control, case 7 and case 9 is attributable to the slippage of DNA polymerase during amplification. The DNA undigested with methylation-sensitive endonuclease HpaII (HpaII-) produced two primary bands with equal intensity. After HpaII digestion (HpaII+), only a single band was observed in the sample of rectum adenocarcinoma used as positive control (arrow), in contrast, all samples of palmar fibromatosis presented two primary allelic bands (arrow), and there is no distinct reduction in the intensity of one of the alleles analyzed by GeneTool Analysis Software. Each of the eight patients had random inactivation of X-chromosome, which indicated palmar fibromatosis is polyclonal.

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