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Table 4 ESC harvesting efficiency with or without feeder cell layers

From: Laser-assisted blastocyst dissection and subsequent cultivation of embryonic stem cells in a serum/cell free culture system: applications and preliminary results in a murine model

No of (%)

Feeder

Without feeder

Isolated ICMs

65

66

Attached

60 (92.3)

54 (81.8)

First dissociation

27 (41.5)

21 (31.8)

   ESC colonies

15 (23.1)a

6 (9.1)b

  1. a,bχ2, 2 × 2, 1 df, Effect of feeder cell on ESC harvesting, p < 0.05
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Journal of Translational Medicine

ISSN: 1479-5876

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