From: Laser-assisted blastocyst dissection and subsequent cultivation of embryonic stem cells in a serum/cell free culture system: applications and preliminary results in a murine model
No of (%)
Feeder
Without feeder
Isolated ICMs
65
66
Attached
60 (92.3)
54 (81.8)
First dissociation
27 (41.5)
21 (31.8)
   ESC colonies
15 (23.1)a
6 (9.1)b