Laser-assisted blastocyst dissection and subsequent cultivation of embryonic stem cells in a serum/cell free culture system: applications and preliminary results in a murine model

Table 3 ESC marker expression in derived ESC lines

			Stemness phenotype*
ESC line ID (Experiment# _Blastocyst#)	Blastocyst manipulation methods	Initial grading	AP activity^≸	Oct-4^≹	TROMA-1^∫
16₇	I	Poor	+	+	-
16₃₄	I	Average	+	+	-
16₃₅	I	Good	+	+	-
17₆	ZF	Poor	+	+	-
17₁₃	IS	Average	+	+	-

* + and – indicate "positive" or "not detectable" activity or protein expression, respectively
^†AP activity is positive marker for pluripotent cells
^‡Oct-4, Octamer-binding transcriptional factor 4, is positive marker for pluripotent cells
^∫ TROMA-1, trophectodermal monoclonal antibody, which reacts to keratin filaments in trophectodermal and endodermal cells, is assessed as a negative marker for pluripotent cells

ISSN: 1479-5876