Skip to main content

Table 2 Influence of blastocyst manipulation on ICM attachment and ESC harvesting

From: Laser-assisted blastocyst dissection and subsequent cultivation of embryonic stem cells in a serum/cell free culture system: applications and preliminary results in a murine model

 

Blastocyst manipulation methods

No. of (%)

IC

ZF

IS

LD

Blastocysts/ICM

25

14

24

21

Attached on MEF

17 (68.0)a

12 (85.7)a,b

24 (100)b

20 (95.2)b

First dissociation

6 (24.0)

3 (21.4)

9 (37.5)

9 (42.8)

ESC primary colonies

4 (16.0)

1 (7.1)

3 (12.5)

5 (23.8)

  1. IC = Intact blastocyst, ZF = Zona-Free blastocyst, IS = Immunosurgery, LD = Laser dissection
  2. a,bχ2, 2 × 4, 3 df, Effect of blastocyst manipulation on ICM attachment, (IC vs IS & LD), p < 0.05
Back to article page