Table 1 Intact blastocyst culture on MEF in different media
Embryonic day (d) | Plating day (D) | No. of (%) | DMEM + 10%NCS, 10% FBS | Ko-M + 15% Ko-S |
|---|---|---|---|---|
d4 | D0 | Blastocysts (I) plated | 63 | 63 |
d5–6 | D1–2 | Hatched blastocysts | 53 (84.1)a | 42 (66.7)b |
d6–7 | D2–3 | Attached on MEF | 51 (80.9) | 42 (66.7) |
d8–9 | D4–5 | ICM isolation | 18 (28.6) | 14 (22.2) |
d14–16 | D10–12 | ESC primary colonies | 1 (1.6)c | 11 (17.5)d |