Figure 2

Laser dissection. Blastocyst secured by two holding pipettes with inner cell mass (ICM) being positioned at 9 o'clock before (a) and after (b) being sectioned by laser with (b) and without (c) zona pellucida. Arrows (b, c) indicate the resected area by laser energy. The smaller blastocyst fragment (white arrow) contains the ICM while the larger (yellow arrow) is exclusively trophoblast (d). Scale = 30 μm.