An alternative flow cytometry strategy for peripheral blood dendritic cell enumeration in the setting of repetitive GM-CSF dosing

Table 4

Antibody cocktails	Complicating factors & potential drawbacks
Lin1 (BD Biosciences^®) CD3, CD14, CD16, CD19, CD20, CD56 negative: MHC class II positive.	Low-level expression of CD14 by "immature" DCs or pDC1 [2, 3] Expression of CD16 by a subset of DCs [3, 39, 40]
CD14, CD16 negative: MHC class II, CD33 positive [56, 57]	Low-level expression of CD14 by "immature" DCs or pDC1 [2, 3] Expression of CD16 by a subset of DCs [3, 39, 40] Expression pattern of CD33 [62]
*Single Antibodies*
BDCA1, BDCA3 (Miltenyi Biotech^®)	Identifies a limited subsets of myeloid DCs, CD1c positive subset (BDCA1) or CD141 expressing subset (BDCA3) [3, 63]
CMRF clones [3, 64]	Identify limited subsets of circulating DCs [3, 64]

ISSN: 1479-5876