An alternative flow cytometry strategy for peripheral blood dendritic cell enumeration in the setting of repetitive GM-CSF dosing

Table 3 Expression of select markers on populations categorized by lineage cocktail reactivity and MHC class II expression. This table lists the percentage of nucleated cells residing in each designated gate for each of the two lineage (Lin) cocktails. The first data column, "Total", represents the total percentage of cells within the designated gates described in the far left hand column. Subsequent data columns denote the percentage of cells residing within the designated gate expressing the designated cell surface molecule designated in the top row. Numbers in parentheses represent the percentage molecule expressing cells in the sample; the "ungated" value represents the total percentage.

Lineage cocktail & gate	Percentage of cells in designated FL-1 Fl-2 gate (portion of ungated marker + population)
	Total	CD11c +	CD14 +	CD80 +	CD83 +	CD86 +
CD14 cocktail
Ungated		(25)	(11.4)	(3.9)	(3.4)	(5.3)
Lin – MHC II -	50.2	2.9 (1.4)	2.5 (1.3)	0 (0)	0 (0)	0 (0)
Lin + MHC II -	13.5	0.6 (0.1)	2.0 (0.3)	0 (0)	0 (0)	0 (0)
Lin + MHC II +	12.9	82.7 (10.6)	72.0 (9.3)	30.5 (3.9)	25.6 (3.3)	39.1 (5.0)
Lin – MHC II +	23.4	52.8 (12.3)	2.5 (0.6)	0.04 (0.01)	0.4 (0.1)	0.9 (0.3)
Gate "B"	6.9	67.9 (4.7)	4.6 (0.3)	0.1 (0.01)	0 (0)	0.15 (0.01)
Gate "C"	0.11	66.7 (0.1)	18.2 (0.02)	10 (0.01)	0 (0)	0.06 (0)
CD66 cocktail
Ungated		(23.8)	(12.3)	(3.1)	(2.8)	(5.8)
Lin – MHC II-	12.9	1.7 (0.2)	0.2 (0.02)	0.08 (0.01)	0 (0)	0 (0)
Lin + MHC II -	52	3 (1.6)	1.1 (0.6)	0 (0)	0.02 (0.01)	0 (0)
Lin + MHC II +	28.7	66.8 (19.2)	32.5 (9.3)	10.4 (3.0)	9.9 (2.8)	18.3 (5.3)
Lin – MHC II +	6.4	44 (2.8)	36.9 (2.4)	1.58 (0.1)	1.25 (0.01)	8.5 (0.5)
Gate "B"	3.8	68.8 (2.6)	61.3 (2.3)	2.7 (0.1)	1.9 (0.01)	14.4 (0.5)
Gate "C"	1.4	95 (1.3)	89.9 (1.3)	7 (0.1)	5.7 (0.01)	27.4 (0.4)

ISSN: 1479-5876