Figure 4

GIM can mediate phagocytosis. Cells were incubated with opsonized fluorescent beads and analyzed using fluorescence microscopy at 20× magnification. A, GIM containing phagocytosed beads. B, Control tumor cell that has fluorescent beads sticking to the surface but not internalized. Cells were analyzed through a z-stack to determine whether the beads were internalized or merely on the surface.