GIM express TLRs. GIM purity was determined as in Fig. 1 and the cells were double-stained for CD45 and TLRs. All CD45+ expressing cells were gated and observed for TLR expression. In each histogram, the data plot on the left indicates the isotype control, and the second plot represents CD45+ gated cells that expressed the respective TLR. These data are from one tumor specimen but are representative of at least five tumor tissue samples from human glioma patients.