|
Age of spheroids in days
|
Radius (μm)
|
Av. volume × 10-6(μm3)
|
Avg. No. of cells/spheroid
|
Avg. Cellular Diameter (μm)
|
Cell packing density
|
Viability (%)
|
Plating Efficiency (%)
|
|---|
|
7
|
93 ± 5
|
3244
|
1176 ± 39
|
13 ± 1
|
0.36
|
98 ± 1.4
|
65 ± 0.6
|
|
14
|
148 ± 10
|
13300
|
2222 ± 47
|
7 ± 1(20%); 14 ± 1
|
0.17
|
78 ± 1.9
|
63 ± 0.2
|
|
21
|
157 ± 11
|
15896
|
4236 ± 63
|
10 ± 1 (25%); 16 ± 1 (63%); 24 ± 2 (12%)
|
0.27
|
77 ± 1.7
|
45 ± 0.2
|
|
28
|
242 ± 19
|
57891
|
8975 ± 78
|
19 ± 2
|
0.16
|
70 ± 3.2
|
25 ± 0.1
|
- Note: All values are mean ± SD of 20–50 observations from each experiment (radius, surface area, volume, Number of cells/spheroid and average size of cells) or 6 observations (viability and plating efficiency) from three experiments.
