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Table 1 Human melanoma cell adhesion to immobilized CD63 mAbs

From: Protein kinase activity is associated with CD63 in melanoma cells

mAba

Specificity

Cell adhesion (%)b

AHN-16

CD63

59

AHN-16.1

CD63

29

AHN-16.2

CD63

55

AHN-16.3

CD63

63

AHN-16.5

CD63

29

U131

not clustered

3

IgGc

N/A

4

9.2.27

MCSP

88

  1. a. Each mAb was coated on plates at a concentration of 1 ug/ml.
  2. b. Assays of adhesion of A375SM melanoma cells to mAbs immobilized on plastic were performed for 30 min. as described in the text. Cell adhesion is expressed as the percent of the total number of cells added. Values are the means of four replicates that agreed within 10 %. A duplicate experiment gave similar results. c. Normal mouse IgG